tmb microwell substrates Search Results


tmb substrate  (Surmodics IVD)
95
Surmodics IVD tmb substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc13106428-247-4-6?v=Surmodics+IVD
Average 95 stars, based on 1 article reviews
tmb substrate - by Bioz Stars, 2026-08
95/100 stars
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tmb 1 component hrp microwell substrate  (ImmunoChemistry Technologies)
93
ImmunoChemistry Technologies tmb 1 component hrp microwell substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb 1 Component Hrp Microwell Substrate, supplied by ImmunoChemistry Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc12006086-60-3-8?v=ImmunoChemistry+Technologies
Average 93 stars, based on 1 article reviews
tmb 1 component hrp microwell substrate - by Bioz Stars, 2026-08
93/100 stars
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tmb one component microwell substrate  (SouthernBiotech)
93
SouthernBiotech tmb one component microwell substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb One Component Microwell Substrate, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/us11278608-666-0-8?v=SouthernBiotech
Average 93 stars, based on 1 article reviews
tmb one component microwell substrate - by Bioz Stars, 2026-08
93/100 stars
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tetramethylbenzidine substrate  (Surmodics IVD)
94
Surmodics IVD tetramethylbenzidine substrate
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tetramethylbenzidine Substrate, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/10__51585_slash_gjvr__2026__1__0180-137-4-16?v=Surmodics+IVD
Average 94 stars, based on 1 article reviews
tetramethylbenzidine substrate - by Bioz Stars, 2026-08
94/100 stars
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tmb high sensitivity substrate solution  (Revvity)
91
Revvity tmb high sensitivity substrate solution
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb High Sensitivity Substrate Solution, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/med_rxiv__2023__05__21__23290299-182-8-13?v=Revvity
Average 91 stars, based on 1 article reviews
tmb high sensitivity substrate solution - by Bioz Stars, 2026-08
91/100 stars
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lstp 1000 01  (Surmodics IVD)
94
Surmodics IVD lstp 1000 01
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Lstp 1000 01, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc06667498-80-43-37?v=Surmodics+IVD
Average 94 stars, based on 1 article reviews
lstp 1000 01 - by Bioz Stars, 2026-08
94/100 stars
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stop solution  (Surmodics IVD)
92
Surmodics IVD stop solution
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Stop Solution, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc11788166-97-3-8?v=Surmodics+IVD
Average 92 stars, based on 1 article reviews
stop solution - by Bioz Stars, 2026-08
92/100 stars
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tmbx surmodics cat no tmbx 1000 01  (Surmodics IVD)
94
Surmodics IVD tmbx surmodics cat no tmbx 1000 01
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmbx Surmodics Cat No Tmbx 1000 01, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc07814939-389-15-16?v=Surmodics+IVD
Average 94 stars, based on 1 article reviews
tmbx surmodics cat no tmbx 1000 01 - by Bioz Stars, 2026-08
94/100 stars
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liquid stop solution for tmb  (Surmodics IVD)
94
Surmodics IVD liquid stop solution for tmb
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Liquid Stop Solution For Tmb, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/pmc11853013-533-63-68?v=Surmodics+IVD
Average 94 stars, based on 1 article reviews
liquid stop solution for tmb - by Bioz Stars, 2026-08
94/100 stars
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tmb microwell substrates  (Surmodics IVD)
94
Surmodics IVD tmb microwell substrates
CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off <t>with</t> <t>PBST.</t> Captured biotinylated mTNFα was detected with streptavidin-HRP and <t>TMB</t> substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.
Tmb Microwell Substrates, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+microwell+substrates/us09833505-565-11-14?v=Surmodics+IVD
Average 94 stars, based on 1 article reviews
tmb microwell substrates - by Bioz Stars, 2026-08
94/100 stars
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Image Search Results


CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Immunization with detoxified TNFα elicits neutralizing antibodies and ameliorates inflammatory shock and autoimmune arthritis in mice

doi: 10.3389/fimmu.2026.1801223

Figure Lengend Snippet: CP-m87S-NH vaccination induces antigen-specific antibodies that block mTNFα binding to TNF receptor. (A) Immunization scheme. ICR mice were immunized intramuscularly with mTNFα vaccines (2 µg antigen) on days 0 and 14, and serum was collected on day 28. (B) Anti-mTNFα IgG titer measured by ELISA. Groups included untreated (n=3), Alum/m87S-NH (n=3), 2HPQ/m87S-NH (n=3), CPQ/m87S-NH (n=3), CP/m87S-NH (n=3), and CP/mTNF-NH (n=3). (C) mTNFα-hTNFR1 binding inhibition (sera diluted 1:15 in PBS) and (D) corresponding inhibition titers. hTNFR1-coated plates were incubated with immunized serum pre-mixed with biotinylated mTNFα at 37 °C for 1 h. After transfer to the hTNFR1-coated plate and incubated for 30 minutes at 37 °C, unbound proteins were washed off with PBST. Captured biotinylated mTNFα was detected with streptavidin-HRP and TMB substrate. Lines and bar graphs show mean ± SD for n=3 mice per group. Statistical significance was determined by one-way ANOVA followed by Tukey’s test. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.001.

Article Snippet: After washing with PBST, TMB substrate (Surmodics, Cat# TMBW-1000-01) was added.

Techniques: Blocking Assay, Binding Assay, Vaccines, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation